Chloroquine cell culture

Discussion in 'Trusted Online Pharmacy' started by karaboz, 07-Mar-2020.

  1. Alexander Halk Moderator

    Chloroquine cell culture


    No effective prophylactic or post-exposure therapy is currently available. We report, however, that chloroquine has strong antiviral effects on SARS-Co V infection of primate cells.

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    The combination of chloroquine with zinc enhanced chloroquine’s cytotoxicity and induced apoptosis in A2780 cells. Thus chloroquine is a zinc ionophore, a property that may contribute to chloroquine’s anticancer activity. Chloroquine inhibits the intracellular multiplication of Legionella pneumophila by limiting the availability of iron. Chloroquine is an inhibitor of the lysosomal degradation of the DNA which is taken up by the cells, so as leelee said, transfection should have been successful, albeit at a slightly lower level than if you would have added the chloroquine. In my normal transfections, I never add chloroquine and get high efficiencies nevertheless. In a three-page paper published in Cell Research, scientists at the Wuhan Institute of Virology’s State Key Laboratory of Virology wrote that both Chloroquine and the antiviral Remdesivir were, individually, “highly effective” at inhibiting replication of the novel coronavirus in cell culture.

    In addition to the well-known functions of chloroquine such as elevations of endosomal p H, the drug appears to interfere with terminal glycosylation of the cellular receptor, angiotensin-converting enzyme 2. These inhibitory effects are observed when the cells are treated with the drug either before or after exposure to the virus, suggesting both prophylactic and therapeutic advantage.

    Chloroquine cell culture

    Chloroquine-induced autophagic vacuole accumulation and cell death in., Chloroquine use in transfection. - Tissue and Cell Culture

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  5. Similar to bafilomycin, the former malaria drug chloroquine CQ is now widely used as an inhibitor of autophagy in both cell culture and in vivo. Chloroquine has a long history of human use and is currently being tested as a sensitizing agent for certain cancers, making understanding its mechanisms of action both topical and important.

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    The conclusion that chloroquine is a zinc ionophore is based on the detection of significantly elevated intracellular zinc levels when both zinc and chloroquine were added to the cell culture medium. The fluorescent zinc probe used in this study is a well-established intracellular marker for zinc ions and has been validated in previous studies. Similar to bafilomycin, the former malaria drug chloroquine CQ is now widely used as an inhibitor of autophagy in both cell culture and in vivo. Chloroquine has a long history of human use and is currently being tested as a sensitizing agent for certain cancers, making understanding its mechanisms of action both topical and important 7, 8, 9. Malaria. Chloroquine has long been used in the treatment or prevention of malaria from Plasmodium vivax, P. ovale, and P. malariae, excluding the malaria parasite Plasmodium falciparum, for it started to develop widespread resistance to it. Chloroquine has been extensively used in mass drug administrations.

     
  6. xinter User

    Plaquenil is the brand name for the prescription drug hydroxychloroquine. Hydroxychloroquine - Wikipedia Plaquenil Side Effects Common, Severe, Long Term - Hydroxychloroquine Side Effects, Dosage, Uses, and More
     
  7. Agnes Moderator

    2019-02-22 - 简书 MM chloroquine diphosphate. Dissolve 0.129 g of chloroquine diphosphate salt in 10 mL of sterile water. Filter sterilize through a 0.22 μm filter. Aliquot 50-100 μL and store at -20 ℃. Aliquots can be thawed and stored at 4 ℃ prior to use. Thawed aliquots should be discarded after 1-2 months. 1 mg/mL PEI, linear MW 25,000 Da

    Chloroquine is a potent inhibitor of SARS coronavirus.